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专利名称：PURIFICATION OF OLIGONUCLEOTIDES发明人：LANGE, Meinolf,GRÖSSEL, Olaf,LINK,
Fritz,SCHÖNBERGER, Andreas,HOHLFELD,Andreas
申请号：EP06707883.2申请日：20060130公开号：EP18431A1公开日：20071024
摘要：A method for purifying a protected oligonucleotide comprising the steps of: a) -providing a solution of the protected oligonucleotide in at least one solvent A having aboiling point below the boiling point of a solvent B, - heating the solution at atemperature of at least 30°C and below the boiling point of the at least solvent A, -adding solvent B until precipitation of a material is visible in the solution, said solvent Bbeing an alcohol having 1 to 6 C-atoms or a diol having 2 to 6 C-atoms, - allowing thesolution to cool down under stirring until formation of a supernatant and a residue, -removing the supernatant or b) - providing solvent B, said solvent B being an alcoholhaving 1 to 6 C- atoms or a diol having 2 to 6 C-atoms, - heating solvent B at a
temperature above 30°C and below the boiling point of solvent B, - adding a solution of aprotected oligonucleotide in at least one solvent A until precipitation of a material isvisible in the solution, - allowing the solution to cool down under stirring until formationof a supernatant and a residue, - removing the supernatant.
申请人：Girindus AG
地址：Buchenallee 20 51427 Bergisch-Gladbach DE
国籍：DE
代理机构：Mross, Stefan P.M.
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